Author Archives: the ligand binding-assay.com team

MicroRNA (miRNA) in Human Mitochondria

Our bioanalytical paper of the month May 2011 is published by Barrey E et al (2011): Pre-microRNA and mature microRNA in human mitochondria. PLoS ONE 6(5), e20220. The authors used supramagnetic microbeads for the isolation of intact mitochondria from human … Continue reading

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The Proximity Ligation Assay – Proximal to Ultimative Assay Sensitivity?

yesterday we had the Ascension Day, also called the Father´s Day in Germany, which means all fathers enjoyed the day along with their kids outside under the fantastic blue sky! And that´s what I did, and got a slight sunborn … Continue reading

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The Invention of Immuno RT-qPCR is in Stand-By Position

RT in this context means “reverse transcription”, not “real-time” as recently suggested (1). When I skimmed through the immuno qPCR publications on Pubmed, I was wondering a bit why nobody have been dealing with reverse-transcription to further increase the sensitivity … Continue reading

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The SELEX Assay – Beyond The Right Track

Why has the systematic evolution of ligands by exponential enrichment (SELEX) become so unpopular? What is the reason why everybody puts much more resources on peptide and antibody based drugs rather than small RNA or small DNA aptamers? We strongly … Continue reading

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Immuno qPCR Where Other Assays Flop

Dear all, after some time of relaxing, we are now proud to provide another exciting ligand binding assay: the immuno qPCR as ultra-sensitive tool for protein or peptide ligand detection. This assay comes both in the microplate and magnetic bead … Continue reading

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